TABLE 1.

Quantitative real-time RT-PCR analysis of MHC-I and type I IFN gene expression

MEF type	Fold change (×10)a,b,c
	H-2Kb	H-2Db	IFN-α	IFN-β
WT	16.2 ± 1.7	15.7 ± 0.7	191.0 ± 36.7	545.8 ± 35.6
IKK1−/−	16.2 ± 2.3	13.5 ± 1.5	187.9 ± 34.0	525.5 ± 14.4
IKK2−/−	13.0 ± 2.3	14.3 ± 1.7	50.2 ± 8.8	81.7 ± 9.1
IKK2*d	NDe	ND	128.8 ± 8.6	422.9 ± 40.0
NEMO−/−	16.4 ± 2.8	14.7 ± 2.9	38.3 ± 5.0	75.2 ± 11.3
IKK1−/− IKK2−/−	14.4 ± 3.0	13.5 ± 0.5	47.9 ± 7.9	80.5 ± 10.1
RelA−/− cRel−/− p50−/−	17.5 ± 0.8	16.0 ± 1.3	44.3 ± 11.1	81.5 ± 17.6
IFNAR−/−	0.7 ± 0.0	0.4 ± 0.1	0.6 ± 0.2	45.9 ± 7.1

^aFold change is represented as mean ± standard deviation of three independent PCR analyses of the same cDNA sample used for Fig. 4 and 5.

^bH-2 gene expression was analyzed at 36 h p.i.

^cIFN gene expression was analyzed at 24 h p.i.

^dIKK2^−/− cells reconstituted with wild-type IKK2.

^eND, not done.