TABLE 2.

Naturally occurring substitutions in 1311 HCV E2 protein sequences

Position	JFH1 residue	Varianta	No.b	Totalc	Prop.d	Accession no. (genotype)e
415	N	D	1	37	0.03	EU482838 (1a)
416	T	A	13	182	0.07	AY956468 (1a), AY958005 (3a), EU155215 (1a), EU155249 (1a), EU155282 (1a), EU155285 (1a), EU155288 (1a), EU155379 (1a), EU255930 (1a), EU255980 (1a), EU482845 (1a), EU482836 (1a), EU643835 (6)
417	N	S	17	27	0.63	EU256046 (1a), EU256031 (1a), EU255964 (1a), EU255952 (1a), EU255943 (1a), EU155354 (1a), EU155347 (1a), EU155297 (1a), EU155274 (1a), EU155215 (1a), EF407468 (1b), EF407466 (1a), EF407477 (1a), EU407415 (1a), EF026073 (2/5 natural recombinant), AY957988 (3a), AM408911 (2/5)
418	G	D	0	2	0.00
422	I	L	5	38	0.13	AB047643 (2a), AF271632 (1a), FJ828970 (1a), FJ828971 (1a), M62321 (1a)

^aThat is, substitutions as described in the text.

^bThat is, the total number of sequences in a sample of 1,311 HCV E2 proteins with the same substitution.

^cThat is, the total number of substituted residues in the sample of 1,311 (see Table 3).

^dProp., the proportion of naturally occurring substitutions that are identical to the substitutions produced in the present study.

^eThe accession number(s) of HCV sequences (genotypes are shown in parentheses) carrying the relevant variant is listed.