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Indirect immunofluorescence analysis of H751JFH1/Zeo cells. (A and B) H751JFH1/Zeo cells (A) and parental Huh7.5.1 cells (B) were immunostained with an anti-NS5A antibody. (C to F) The subcellular colocalization of de novo-synthesized HCV RNA and NS5A in H751JFH1/Zeo cells was analyzed. The cells were stained with DAPI (C), an anti-bromodeoxyuridine antibody (D), and an anti-NS5A antibody (E). The merge panel is shown in panel F.
