FIG. 4.
Lentiviral transduction of DC results in the upregulation of NF-κB, AP-1, and IRF. At day 4, DC were plated at 106 DC/ml medium and transduced with lentiviral vectors encoding cherry fluorescent protein or GFP under the transcriptional control of NF-κB or the latter together with AP-1 and IRF3 (IFN-β promoter) (n = 3). (a and c) Graphs representing the flow cytometry data of a representative experiment demonstrating the relative upregulation of reporter expression (fold increase compared to background) driven by the NF-κB and the IFN-β promoters, respectively. Stimulation with LPS (white bars), active reverse transcriptase (black bars), and inactive reverse transcriptase (gray bars) lentiviral vectors is depicted. (b and d) Confocal images representative of the data obtained with the NF-κB (b) and IFN-β (d) promoters.