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. 2010 Mar 17;84(11):5627–5636. doi: 10.1128/JVI.00014-10

FIG. 5.

FIG. 5.

DC activation by lentiviral vectors is dependent on both TRIF and MyD88. DC of wild-type, TRIF−/−, MyD88−/−, or TRIF/MyD88−/− mice were manipulated as described in the legend of Fig. 1 (n = 3). (a) Graph showing the upregulation of CD86 upon stimulation with LPS (black bars) or transduction with lentiviral vectors (white bars) of wild-type (WT), TRIF−/−, and MyD88−/− DC. The data shown are representative of data from three independent experiments. (b) Flow cytometry graphs representing TRIF/MyD88−/− DC, which were not stimulated (black lines) or stimulated with lentiviral vectors (red lines) or TNF-α (blue lines). The percentage of marker-positive cells is indicated. The data are representative of data from three independent experiments. (c) Graphs showing a summary of the TNF-α and IFN-β secretion by wild-type (white bars), MyD88−/− (black bars), TRIF−/− (dark gray bars), and TRIF/MyD88−/− (light gray bars) DC (n = 3). KO, knockout; DKO, double knockout.