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. 2010 Apr 14;84(12):5947–5957. doi: 10.1128/JVI.02575-09

FIG. 6.

FIG. 6.

Slot blot analysis of cells after transfection with or without synthesized viral RNA or after infection with or without viruses. (A) HeLa cells were transfected with or without (Mock) RNAs of pOM1, pOMΔ0.8, pOMΔ1.8, pOMΔP1, pOMΔP1Δ2A, or pOMΔP1Δ2AΔ2B, and cell lysates were collected 2 and 8 h later. The RNAs were detected with probes for the PV IRES sequence or GAPDH sequence. (B) A similar experiment was performed using the RNAs of pOM1, pOMΔP1, pOMΔP1Δ2A, pOMΔP1Δ2AΔ2B, pOM-EGFPΔP1, and pOM-EGFPΔP1Δ2A. (C) HeLa cells were infected with or without (Mock) PV1(M)OM, OMΔ0.8, OMΔ1.8, OMΔP1, or OMΔP1Δ2A, and cell lysates were collected 2 and 8 h later. The RNAs were detected with probes for the PV IRES sequence or GAPDH sequence. Relative amounts of PV RNA corrected to the amount of GAPDH RNA are shown.