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P1-null PV vector with or without the 2Apro coding region expresses EGFP. HeLa cells were mock infected (c, f, and i) or infected with OM-EGFPΔP1 (a, d, and g) or OM-EGFPΔP1Δ2A (b, e, and h) and observed 24 h later under a fluorescence microscope. Upper panels show fluorescence (FL) images (a to c), panels in the middle show bright-field (BF) images (d to f), and lower panels show merged (FL+BF) images (g to i). Bar, 100 μm.
