FIG. 2.
Generation of virus from cDNA clones in RD cells. (A) Flow cytometric analysis of CAR and DAF expression on RD cells. For detection of CAR and DAF, anti-CAR (RmcB) and anti-DAF (BRIC110) monoclonal antibodies were used (black histograms). A mouse IgG1 antibody was used as a negative control (gray histograms). (B) Total virus yield (extracellular and intracellular) determined for indicated viral variants by a TCID50 assay in GMK cells. Viruses were generated from infectious cDNA clones by transfection and one passage in RD cells (96 h p.i.). Error bars represent mean ± SEM of triplicate samples.