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. 2010 Apr 7;84(12):6007–6017. doi: 10.1128/JVI.00398-10

FIG. 1.

FIG. 1.

Structure and in vitro characterization of therapeutic bicistronic HC-Ad. (A) Structure and transcriptional regulation of HC-Ad-TK/TetOn-Flt3L. (B) CNS1 GBM cells and rat astrocytes in primary culture were infected with HC-Ad-TK/TetOn-Flt3L with or without the inducer doxycycline (Dox). Flt3L expression was assessed by immunofluorescence and ELISA. *, P < 0.05 versus mock and infected controls, by one-way ANOVA followed by Tukey's test. (C) CNS1 GBM cells and astrocytes in primary culture were infected with the bicistronic HC-Ad and incubated with or without the prodrug GCV. TK expression was assessed by immunofluorescence, and cell death was determined by flow-cytometric analysis of annexin V/propidium iodide-stained cells as shown in representative dot plots. *, P < 0.05 versus mock and infected controls, by one-way ANOVA followed by Tukey's test.