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. 2010 Apr 7;84(12):6163–6176. doi: 10.1128/JVI.00331-10

FIG. 3.

FIG. 3.

MNV-1 infection requires dynamin II. RAW 264.7 cells (A) and BMDMs (B) were pretreated with dynasore at the indicated concentration for 30 min, infected with MNV-1 or VSV on ice for 1 h, and washed with PBS. At 8 h (RAW 264.7 cells) or 10 h (BMDMs) postinfection, cells were freeze-thawed two times and viral titers determined by plaque assay. (C) RAW 264.7 cells were transfected with a wt and DN construct of GFP-tagged dynamin II and then infected with MNV-1 (MOI of 10) for 12 h. The number of VPg-expressing cells was determined by immunofluorescence and normalized to the wt control. (D) RAW 264.7 cells were infected with NR-containing virus at an MOI of 0.001, with rocking at room temperature for 60 min. RAW 264.7 cells were either pretreated (pretreatment) or treated for 60 min postinfection (posttreatment) with 80 μM dynasore before performing a plaque assay and determining viral titers. *, P < 0.05; ***, P < 0.001.