TABLE 1.
FACS analysis of the cell cycle distribution of H-1PV-infected HEK-293 cellsa
| Type of infected cell | Subpopulation size (%) at indicated phase and time postinfection (h) |
||||||||
|---|---|---|---|---|---|---|---|---|---|
| G1 |
S |
G2 |
|||||||
| 24 | 48 | 72 | 24 | 48 | 72 | 24 | 48 | 72 | |
| Mock | 46.04 ± 0.33 | 46.52 ± 0.64 | 48.23 ± 1.74 | 33.05 ± 3.48 | 32.6 ± 2.46 | 33.58 ± 2.53 | 20.92 ± 3.46 | 20.87 ± 1.83 | 17.18 ± 0.52 |
| H-1PV | |||||||||
| ΜΟI of 1 PFU/cell | NA | NA | 34.96 ± 0.96 | NA | NA | 21.49 ± 4.46 | NA | NA | 43.54 ± 3.5 |
| ΜΟI of 10 PFU/cell | 19.5 ± 2.28 | 22.75 ± 2.04 | 19.81 ± 0.92 | 23.86 ± 5.91 | 17.59 ± 3.38 | 14.24 ± 3.94 | 56.64 ± 3.67 | 59.66 ± 2.04 | 65.73 ± 5.14 |
| UV-treated H-1PV | |||||||||
| ΜΟI of 10 PFU/cell | 49.14 ± 0.43 | 47.65 ± 1.05 | 45.94 ± 1.33 | 29.41 ± 2.15 | 31.75 ± 2.28 | 35.01 ± 0.4 | 21.45 ± 1.74 | 20.6 ± 1.54 | 19.05 ± 1.32 |
| ΜΟI of 30 PFU/cell | NA | NA | 43.1 ± 2.17 | NA | NA | 36.54 ± 2.43 | NA | NA | 20.36 ± 0.31 |
| ΜΟI of 100 PFU/cell | NA | NA | 44.6 ± 2.24 | NA | NA | 36.31 ± 0.91 | NA | NA | 19.1 ± 1.33 |
a
Cultures were infected at different MOI and analyzed at the indicated times postinfection. NA = data not available.