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. 2010 Mar 31;84(12):5958–5974. doi: 10.1128/JVI.00161-10

FIG. 4.

FIG. 4.

Reactivation of latent HIV-1 in primary memory CD4+ T cells. (A) After 14 days of culturing in quiescent conditions (and 28 days after CD4+ T-cell isolation), the cells were treated with antilatency drugs HMBA (H), prostratin (P), SAHA (S), or the combination of prostratin and SAHA (P+S). The data shown include each LGIT subtype for each of three independent donors. Asterisks indicate synergism of the prostratin-SAHA combination with respect to either drug alone. For details on the quantitative treatment of synergy, see Materials and Methods. (B to D) At day 21 postinfection (day 28 postisolation), primary cells were examined for the proviral LTR expression of GFP (x axis) and the surface expression (y axis) of CD4 (B), CD45RO (C), or CD27 (D). (E to G) At days 21 postinfection (same as panels B to D), primary cells were treated with prostratin and SAHA for 24 h and examined for proviral LTR expression of GFP (x axis) and surface expression (y axis) of CD4 (E), CD45RO (F), or CD27 (G).