FIG. 7.

Effects of polymorphisms at BAFF-R on FV-neutralizing Ab production and plasma viremia. (A) Linkage mapping of the Rfv3 gene by using (B10.A × A/WySn) × A/WySn backcross mice (16). The genotypes of APOBEC3 (d, A/WySn derived; b, B10.A derived) and BAFF-R (mu, A/WySn derived; wt, B10.A derived) for eight mice that possessed a recombination at the indicated region in chromosome 15 were determined by genome sequencing and PCR-based analyses, respectively,. The neutralizing Ab titers of these mice infected with 150 SFFU FV are shown at the right. (B and C) BAFF-R^−/− and WT B6 mice were infected with 10,000 SFFU FV, and their plasma levels of viremia and virus-neutralizing Ab titers were analyzed by the same methods as described for Fig. 1. (D and E) A/WySn and A/J mice were infected with 1,500 SFFU FV, and the neutralizing Ab titers at PID 14 and PID 28 and plasma viral loads at PID 7 and PID 28 were analyzed. *, P < 0.005; **, P < 0.0005 (by t test).