TABLE 2.
Primers used for chromatin immunoprecipitation and electrophoretic mobility shift experiments
| Position with respect to TSS | Amplified region | ChIP-PCR forward primer | ChIP-PCR reverse primer |
|---|---|---|---|
| −56 | −120 to 0 | CAGAATAACGGGTGGAAATG | TGGCCAGACAGCTTTCTAAC |
| 90 | 41 to 150 | TGTGCGTGTGGGTGGCATTC | CGAAGATCTCAGATCCCAGT |
| 326 | 276 to 375 | GAGAGCTTCACAGCATGCAC | CTTGAGTGGCTGCGGCTGCG |
| 580 | 521 to 640 | CGACAGCCACAGTTCCCCTG | GTGTTGAATGTGGCTGGACCG |
| 650 | 606 to 710 | GGTGGAGATCCCTCCGGTCC | CTCCAGGAGGAGGGAAAAGG |
| 745 | 701 to 790 | CCTCCTGGAGACGGGGGAGG | GCCCGCCACGTGGGGAGCCC |
| 1233 | 1181 to 1285 | CTTCCCGAAGATCCACCGGC | GGCAGCTGCCGTCCCGGAGC |
| 51200 | 51128 to 51307 | GAGTTATTTGATTTCTTCGTTG | GAAGATGGAAGCAGAACACC |
| 79800 | 79733 to 79903 | GTGTATTGTGTGTGATGCAAG | GTTACTTGAACTCACTCACTTG |
| 95585 | 95538 to 95660 | CTAGTCCCAGAATGTCAGCC | GCCTCTCCCATGTCTCCATG |
| EMSA probes | Designated | ||
| 95585 | 585 | CTGTATGTCATGCTGACCTTATTGTTAAACAC | |
| 95585 mutant | 585μ | CTGTAGATCTTGCGTCTATTATTGTTAAACAC | |
| 95585 core | 585core | CGTGTCATGCTGACCGA | |
| 95585 mutant core | 585μcore | GCAGCTTATGCTGATAG |