TABLE 1.
Yeast strains
| Strain | Genotypea |
|---|---|
| LNY150 | MATa/MATα leu2::hisG/leu2::hisG trp1::hisG/trp1::hisG lys2/lys2 his4-N/his4-G ura3/ura3 ho::LYS2/ho::LYS2 |
| MSY311 | sum1-i::URA3/sum1-i::URA3 cdc28-as1/cdc28-as1 SMK1-HA::KAN/SMK1-HA::KAN |
| MSY312 | cdc28-as1/cdc28-as1 SMK1-HA::KAN/SMK1-HA::KAN |
| MSY334 | SMK1-HA::KAN/SMK1-HA::KAN |
| MSY338 | sum1-ci::URA3/SUM1 SMK1-HA::KAN/SMK1-HA::KAN |
| MSY339 | sum1-ci::URA3/sum1-ci::URA3 SMK1-HA::KAN/SMK1-HA::KAN |
| MSY349 | sum1-c::URA3/sum1-c::URA3 SMK1-HA::KAN/SMK1-HA::KAN |
| MSY352 | sum1-i::URA3/sum1-i::URA 3SMK1-HA::KAN/SMK1-HA::KAN |
| MSY351 | ZIP1::GFP700/zip1::GFP700 ndt80Δ::LEU2/ndt80Δ::LEU2 |
| MSY362 | ZIP1::GFP700/zip1::GFP700 |
| MSY386 | zip1::GFP700/zip1::GFP700 sum1-ci::URA3/sum1-ci::URA3 |
| MSY350 | ndt80::TRP1-PGAL1-NDT80/ndt80::TRP1-PGAL1-NDT80 ura3::PGPD1-GAL4(848)-ER::URA3 |
| MSY385 | ndt80::TRP1-PGAL1-NDT80/ndt80::TRP1-PGAL1-NDT80 ura3::PGPD1-GAL4(848)-ER::URA3 sum1-ci::URA3/sum1-ci::URA3 |
| MSY390 | hst1Δ::KAN/hst1Δ::KAN sum1-ci::URA3/sum1-ci::URA3 |
| MSY391 | hst1Δ::KAN/hst1Δ::KAN |
| MSY392 | sum1-ci::URA3/sum1-ci::URA3 |
| MSY434 | sum1-ci::URA3/sum1-ci::URA3 TRP1-NDT80-trp1::hisG/TRP1-NDT80-trp1::hisG SMK1-HA::KAN/SMK1-HA::KAN |
| MSY428 | sum1-ci::URA3/sum1-ci::URA3 ndt80Δ::LEU2/ndt80Δ::LEU2 TRP1-NDT80-trp1::hisG/TRP1-NDT80-trp1::hisG |
| MSY426 | sum1-ci::URA3/sum1-ci::URA3 ndt80Δ::LEU2/ndt80Δ::LEU2 TRP1-NDT80-trp1::hisG/trp1::hisG |
| MSY438 | sum1-ci::URA3/sum1-ci::URA3 ndt80Δ::LEU2/ndt80Δ::LEU2 TRP1-ndt80-ΔM1-trp1::hisG/TRP1-ndt80-ΔM1-trp1::hisG |
| MSY444 | sum1-ci::URA3/sum1-ci::URA3 TRP1-ndt80-ΔM1-trp1::hisG/TRP1-ndt80-ΔM1-trp1::hisG SMK1-HA::KAN/SMK1-HA::KAN |
| MSY447 | rfm1Δ::KAN/rfm1Δ::KAN |
| MSY448 | rfm1Δ::KAN/rfm1Δ::KAN sum1-ci::URA3/sum1-ci::URA3 |
| MSY458 | ndt80Δ::LEU2/ndt80Δ::LEU2 |
| MSY460 | ndt80Δ::LEU2/ndt80Δ::LEU2 sum1-c::URA3/sum1-c::URA3 |
| EWY167 | sum1-ci::URA3/sum1Δ::KAN |
a
All strains are MATa/MATα diploids in the LNY150 (SK1) genetic background (35). The his4-N or his4-G allelic status of the strains has not been tested unless otherwise indicated.