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. 2010 Apr 5;30(12):2918–2932. doi: 10.1128/MCB.00131-10

FIG. 3.

FIG. 3.

Rescue of Erk2−/− mice from early embryonic lethality by tetraploid aggregation. Each eight-cell-stage embryo isolated from intercrosses of CD-1 Erk2+/− mice was aggregated with two tetraploid four-cell-stage wild-type embryos in vitro. After development to the blastocyst stage, chimeric embryos were transferred into the uteri of pseudopregnant females. (A) Macroscopic views of wild-type and Erk2−/− embryos at E13.5 (upper panels) and contribution of wild-type GFP-positive cells to the placenta and yolk sac endoderm (bottom panels). (B) Genotyping analysis of embryos obtained from tetraploid aggregation experiments. (C) Number and genotype of embryos obtained by tetraploid aggregation rescue experiments at E13.5.