Figure 5.
Effect of pharmacological agents on evoked dopamine concentration. A) Concentration vs time profiles from individual VNCs show that peak dopamine concentration is reduced when larva are chronically fed NSD-1015 (6.6 μM), a synthesis inhibitor, or are incubated in reserpine (100 μM), a VMAT inhibitor that disrupts vesicular packaging. B) The concentration of dopamine elicited by the first stimulation is significantly less for NSD-1015 (** p < 0.01) and reserpine (*** p < 0.001) than in samples dissected in buffer, but more than control experiments where ChR2 was not expressed. * p < 0.05, ** p < 0.01, *** p < 0.001.