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. Author manuscript; available in PMC: 2011 May 1.
Published in final edited form as: Biochem J. 2010 Apr 14;427(3):467–475. doi: 10.1042/BJ20091594

Figure 3. The binding of endostatin to TG-2 is cation-dependent.

Figure 3

A) Solid-phase assays: effect of cations. Binding of human TG-2 (64 nM) to coated endostatin produced in HEK 293 cells (0.1 µg/well) in the presence of cations (8 mM). (mean of 3 experiments ±SD) B) Binding of human TG-2 (0, 1, 2, 4, 8, 16, 32, 64, 128, 256 nM to immobilized human endostatin (––– wild-type endostatin, — D104N mutant endostatin; 0.1 µg/well) in the presence of 8 mM CaCl2. The values are expressed as mean ± SD (n=3).