Table 1. Kinetic and equilibrium dissociation constants of the binding of transglutaminase-2 to endostatin.
Rate and affinity constants of the binding of soluble transglutaminase-2 to immobilized endostatin calculated from SPR binding assays using the Biacore T100 evaluation software 2.0.1.
| Endostatin (Immobilized on the chip) |
HEK 293 EBNA cells |
P. pastoris |
|---|---|---|
| Guinea pig TG-2 Injected in soluble form |
+ 2 mM CaCl2 | + 2 mM CaCl2 |
| ka (M−1s−1) | 1.12×105 M−1 s−1 | 6.10 ×104 M−1 s−1 |
| kd (s−1) | 7.65×10−4 s−1 | 3.41×10−4 s−1 |
| KD (M) | 6.8 nM | 5.59 nM |
| Chi2 | 4.1 | 8.81 |
| U-value | 1 | 1 |