Table 2. Laboratory features of Mycobacterium microti isolates from Scotland*†.
Source | Specimen | Direct AFB | Growth on primary isolation |
Drug susceptibility | Genotype | |||
---|---|---|---|---|---|---|---|---|
Solid culture |
Liquid culture |
|||||||
IUT | PYR | MB/MGIT | ||||||
Human 1 | Sputum | Positive | – | + | – | Failed to grow in liquid cultures | Llama type SIT641 (spacers 4–7, 23,24, 37, and 38) | |
Human 2 | Sputum | Positive (many) | – | + | – | Failed to grow in liquid cultures | Llama type SIT641 | |
Human 3 | Sputum | Positive (many) | – | – | + | Susceptible to R, I, E; resistant to P | Llama type SIT641 | |
Human 4 | Sputum | Positive (few) | + | + | – | Failed to grow in liquid culture | Llama type (spacers 4–7 and 23,24 only) | |
Cat 1 | Tissue/lymph node | Negative | – | + | – | Failed to grow in liquid cultures | Llama type SIT641 | |
Badger | Tissue/lung | Strongly positive | + | + | ± | Inadequate growth | Vole type SIT 539 (spacers 37 and 38) | |
Cat 2 | Tissue/lymph node | Negative | – | + | – | Susceptible to R,I,E,P (grew on liquid subculture) | Vole type SIT539 | |
Llama | Tissue/lung | Positive | + | + | – | Failed to grow in liquid culture | Llama type SIT641 | |
Ferret | Tissue | Positive | ± | ± | ± | Susceptible to R,I,E,P | Not tested |
*AFB, acid-fast bacilli; IUT, International Union Against Tuberculosis formulation of solid egg medium; PYR, IUT medium with pyruvate supplementation; MB, MBBact, Biomerieux, Basingstoke, United Kingdom; MGIT, Mycobacteria Growth Indicator Tube, Becton Dickinson; R,I,E,P, rifampin, isoniazid, ethambutol, pyrazinamide; SIT, Spoligo-International-Type. †SIT numbers: designations in International spoligotyping database (Spol DB4) (14).