Skip to main content
. 2010 Mar 15;588(Pt 9):1435–1449. doi: 10.1113/jphysiol.2010.188318

Figure 4. Temperature dependence of WT (A and B) and HV1ΔC proton currents (C and D) recorded in inside-out patches of membrane.

Figure 4

For each patch, families of pulses to the same voltages were applied, with the pulse duration adjusted as shown. A, WT proton currents during pulses from −60 mV to +20 mV in 10 mV increments at 20.8°C (A) and 31.2°C (B), at pHo 7.5, pHi 6.5. C, HV1ΔC proton currents in a patch elicited by families of pulses from −40 mV to +80 mV in 10 mV increments, at 11.9°C. D, pulses to the same voltages in the same patch at 23.3°C. Both at pHo 7.5, pHi 7.5. E, summary of the temperature dependence of WT and HV1ΔC proton channel gating kinetics and conductance. Measurements were made in excised inside-out patches to avoid depletion effects. Means ±s.e.m. are shown for 6–8 WT (HV1) patches and 6–9 HV1ΔC patches. *P < 0.01 by Student's t test.