Skip to main content
. 2010 Mar 23;19(12):2421–2432. doi: 10.1093/hmg/ddq125

Figure 1.

Figure 1.

NHS localizes to sites of cell contact, the leading edge of lamellipodia and focal adhesions. (A) Endogenous NHS (red) localized to sites of cell–cell contact in Caco-2 cells, detected by an N-terminal isoform-specific antibody (left panel) and a C-terminal pan NHS antibody (right panel). Lower panel for each antibody staining is a higher magnification. Nuclei were counterstained with DAPI. Staining for NHS (red) was prominent at tricellular contacts (arrowheads). Scale bar 10 µm. (B) Endogenous NHS (detected with pan NHS antibody; red) localized at the leading edge of lamellipodia in stimulated MTLn3 cells at the late 3 min transient (arrowheads). Cells were counterstained for F-actin (green). Top panel, unstimulated; middle panel, 3 min stimulation; bottom panel, enlargement of boxed area showing NHS at the leading edge of the lamellipod. Scale bar 10 µm. (C) Live TIRF imaging of GFP-NHS-1A (green) in MCF7 cells revealed NHS localization at cell junctions (arrowhead), the leading edge and co-localization with actin puncta (arrows) and actin fibres (red, top panel). GFP-NHS-1A co-localized with the focal adhesion complex protein Lasp1 (red, bottom two panels). Scale bar 10 µm.