Figure 6.

Cross-linked precursor dimers and tetramers are formed within individual receptor complexes. (A) [³H] tOE17-20F (lane 1), [³H] tOE17-20F3C (lane 2) or [³H] tOE17-20F3C17C (lane 3) precursors were incubated with thylakoids in binding reactions. Recovered thylakoids were solubilized in 1% digitonin and analysed by BN–PAGE and fluorography. Locations of molecular weight markers ferritin monomer and dimer are indicated on the left of the panel. (B) tOE17-20F (lanes 1 and 4), tOE17-20F3C (lanes 2 and 5) or tOE17-20F3C17C (lanes 3 and 6) precursor-bound thylakoids from (A) were either dissolved in urea-SDS sample buffer (lanes 1–3) or first solubilized in buffer containing 1% digitonin and then >24 h later mixed with urea-SDS sample buffer (lanes 4–6). Samples were analysed by non-reducing SDS–PAGE. (C–E) BN–PAGE lanes [³H] tOE17-20F (C), [³H] tOE17-20F3C (D) or [³H] tOE17-20F3C17C (E) were subjected to second dimension SDS–PAGE. An aliquot of respective binding sample in urea-SDS, mixed with molecular weight markers, was run on the left side of the SDS–PAGE gels (lane 1).