Figure 6.

Post-transcriptional tpr gene silencing by RNAi does not impair subsequent PV infection and degradation of nucleoporins. Four days after transfection with Tpr siRNAs or mock transfection with non-target control siRNAs (Ctrl 1), or after incubation with transfection reagent alone (Ctrl 2), cells were either infected with PV or not, and harvested 10 h later. Total cell proteins were analysed by immunoblotting. Regardless of whether Tpr had already been eliminated by RNAi before PV infection or not, Nup153 and Nup98 were again degraded in the infected cells whereas the NPC core protein Nup133 and lamin A remained unaffected. PV-induced degradation of nucleoporins such as Nup35, and stability of others such as Nup107 and gp210, was also similar in control and Tpr-RNAi cells (not shown).