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Nucleotide incorporation at a blunt-end primer template duplex. (A) Partial sequence used in primer extension experiments. (B) Single nucleotide incorporation of KlenTaq by extension of blunt-end primer template duplex for 2, 10, or 60 min, respectively. The respective dNTP is indicated. (C) As in (B) for KlenTaq mutant Tyr671Trp in presence of a blunt-end primer template duplex.
