Figure 1.
Overexpressing Mef2 in clock neurons disrupts normal circadian locomotor activity. A, Representative double-plotted actograms in DD from Mef2EP1751 flies (left column), UAS-Mef2 III(low) flies (center column), and UAS-Mef2 III(high) flies (right column). Actograms are shown for these flies crossed to y w flies (top row), Pdf-Gal4 (center row) and tim(UAS)-Gal4 flies (bottom row). B, Graph shows percentage of flies exhibiting either a single rhythm (gray), complex rhythms (checked) or arrhythmicity (black). A complex rhythm is defined as a rhythm exhibiting more than one significant period above the significance level at 95% confidence when analyzed with Lomb–Scargle analysis. Single rhythm and arrhythmicity are defined by the presence or the absence of a peak above the significance level respectively. I, II, and IV refer to UAS-Mef2 constructs for isoforms A, B, and D, respectively. III(low) and III(high) refer to two different UAS-Mef2 transgenes for Mef2 isoform C that express low and high levels of Mef2 protein respectively.