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. 2010 Apr 28;30(17):5855–5865. doi: 10.1523/JNEUROSCI.2688-09.2010

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Copyright © 2010 the authors 0270-6474/10/305855-11$15.00/0

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Figure 4. — Expression of a Mef2 repressor or knocking down Mef2 expression in clock neurons disrupts circadian behavioral rhythms. A, Representative double-plotted actograms from UAS-Mef2-EnR (left panels) and UAS-Mef2^RNAi (right panels) flies in DD crossed to either y w control flies (top row), Pdf-Gal4 (center panel) and tim(UAS)-Gal4 flies (bottom panels). The Pdf-Gal4 line crossed to UAS-Mef2-EnR had two copies of the Pdf_0.5-Gal4 driver. The Pdf-Gal4 and tim(UAS)-Gal4 flies crossed to UAS-Mef2^RNAi flies also had a UAS-Dcr-2 transgene. B, Representative Lomb–Scargle periodograms for tim(UAS)-Gal4/+; UAS-Dcr-2/UAS-Mef2^RNAi flies from day 1 to 6 (left) and from day 6 to 12 (right) show the decrease in power of the rhythm in the second half of the assay.