Figure 8.
Knocking down Mef2 in clock neurons dampens molecular clock oscillations in s-LNvs. A, B, Time series immunocytochemistry on whole mount brains from either UAS-Mef2RNAi/+ control flies (labeled Con) or tim(UAS)-Gal4/+; UAS-Mef2RNAi/UAS-Dcr-2-2 flies (RNAi). Images show s-LNvs stained with antibodies to PER (red), VRI (blue) and PDF (green). Time series are shown from either the second day (DD2 in A) or the eighth day in DD (DD8, B). These images are representative of at least 10 brains stained in each experiment. Each experiment was performed 3 times with very similar results.