Table 2.
Estimated rate constants for EGFR phosphorylation/dephosphorylation and adaptor recruitment
| Parameter description | Value |
|---|---|
| Receptor tyrosine phosphorylation rates | |
| Residue 992 | 0.055 (nM × s)-1 |
| Residue 1068 | 0.055 (nM × s)-1 |
| Residue 1148 | 0.0063 (nM × s)-1 |
| Residue 1173 | 0.055 (nM × s)-1 |
| Multiplier when a receptor is phosphorylated. | 3 |
| Multiplier when a receptor is ligand-bound. | 2 |
| Receptor tyrosine dephosphorylation rates | |
| Residue 992 | 0.013/s |
| Residue 1068 | 0.013/s |
| Residue 1148 | 0.0014/s |
| Residue 1173 | 0.013/s |
| Multiplier when ligand is removed. | 4 |
| Adaptor docking rates | |
| For Grb2 | 0.0072 (nM × s)-1 |
| For Stat5 | 0.0055 (nM × s)-1 |
| For PLCγ1 | 0.00216 (nM × s)-1 |
| For Shc docking to pY1148 | 0.00936 (nM × s)-1 |
| For Shc docking to pY1173 | 0.0056 (nM × s)-1 |
| Adaptor dissociation rates | |
| For Grb2 | 9.34/s |
| For Stat5 | 11.74/s |
| For PLCγ1 | 21.15/s |
| For Shc dissociation from pY1148 | 13.39/s |
| For Shc dissociation from pY1173 | 13.39/s |
| Kd of adaptor recruitment to EGFR | |
| For Grb2 | 1.3 μM |
| For Stat5 | 2.13 μM |
| For PLCγ1 | 9.8 μM |
| For Shc dissociation from pY1148 | 1.43 μM |
| For Shc dissociation from pY1173 | 2.4 μM |