Table 3.

Binding constants reported in literature

Binding domain	Target	Kd	Kon	Koff	Method	Reference
SHC PTB	EGFR (32 PY peptides)	<2 μM			Protein microarray & SPRa	[26]
	EGFR PY1148 peptide	28 nM			ITCb	[71]
	TRK PY490 peptide	42 nM
SHC SH2	EGFR PY1148 peptide	NBc			SPR	[27]
	EGFR PY1173 peptide	65 nM
PLCγ1 SH2	EGFR PY peptides	<2 μM			Protein microarray	[26]
PLCγ1 N+C SH2	EGFR PY992	200 nM			SPR	[28]
	EGFR PY1173	740 nM
STAT1,3 SH2	EGFR PY peptides	>2 μM			Protein microarray	[26]
Grb2 SH2	EGFR PY1068 peptide	30 nM			SPR	[27]
	EGFR PY1086 peptide	60 nM
	EGFRPY1148 peptide	NB
	EGFR PY1173-7Y4	NB
	EGFR PY992-8Y4	NB
	EGFR phosphopeptides	<2 μM			Protein microarray	[26]
Grb2 protein	EGFR PY1068 peptide	380 nM			ITC, SPR	[31]
	EGFR PY1068 peptide	713 nM			SPR	[29]
	Activated EGFR in A431 membranes (fractional)	97-650 nM	0.016/nM/s (78%)	7.5-8.1/s (89%)	Single molecule analysis	[32]
			0.005/nM/s (21%)	1.6-2.6/s (<11%)
			0.0022/nM/s (1%)	0.07-0.4/s (<5%)
p85 N-SH2	PDGFR PY315 peptide	300 nM			ITC, SPR	[72]
p85 2 SH2	IRS-1 PY	0.3-3.0 nM	0.03-0.4/nM/s	0.11-0.19/s	SPR	[73]
Src SH2	(YMXM) peptides polyoma middle tumor antigen	600 nM			ITC	[74]
Lck SH2	Lck Y505 peptide	4 μM			ITC, SPR	[72]
	EGFR C-terminus	5 μM			ITC	[74]

^aSPR = Surface Plasmon Resonance, ^bITC = Isothermal Titration Calorimetry, ^cNB = No binding