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Expression analysis of genes involved in mammalian brain convolution. mRNA was extracted from pig cortical tissue from E60 and E80. qRT-PCR analysis were performed on cDNA for the genes DCX, ARX, GPR56, FLNA, Reelin, VLDLR, ApoER2, Dab1, FYN, LIS1, NDEL1, and CDK5. The expression levels were normalized to GAPDH, Beta-actin and 18S rRNA expression using the geNorm program [30].
