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. 2010 May 26;5(5):e10841. doi: 10.1371/journal.pone.0010841

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This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

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(a) ChAB increases total cell and DCX+ cell number in a dose-dependent manner, in fetal NSC cultures, in the presence of FGF2 (4-d treatment), as well as (b) following FGF2 withdrawal (6-d FGF2 followed by FGF2 withdrawal and addition of ChAB, for 2-days). (c) Confocal projections of fetal NSC cultures described in (b). (d) Fetal NSC cultures expanded in FGF2 (4-days) and then switched to FGF2+/- CholAB – containing medium exhibit expansion of DCX+ early neurons. (e) Fetal NSC cultures expanded in FGF2 (6-days) and then switched to +/- CholAB conditions, in the absence of FGF2 (for 2-days) exhibit expansion of DCX+ early neurons. [Size bars: 100 µm].