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. 2010 May 26;5(5):e10852. doi: 10.1371/journal.pone.0010852

Figure 1. Creation of Grp94 knockout (KO) and floxed (F/F) mice.

Figure 1

(A) Schematic drawings of the Grp94 exon map, the wild-type (WT) (+) allele, the loxP-FRT targeting vector, the Floxed (F) allele and the KO (−) allele. The ATP-binding domain of GRP94, the insertion positions of neo cassette, the loxP (closed triangle) and FRT (open triangle) sites, and the pgk-TK expression cassette are indicated. The BamHI restriction digestion sites, the position of the 5′-probe for Southern blotting, the expected location and size for both the Southern blot products and the genotyping products are also indicated. (B) Southern blot results of BamHI digested DNA from Grp94 WT (+/+) and heterozygous (+/−) siblings. The size of each band is indicated. (C) Genotyping results of DNA from Grp94+/+ and +/− mice. The sizes of the PCR products are indicated. (D) Genotyping results of DNA from Grp94F/+, +/+ and F/F mice. The sizes of the PCR products are indicated.