Figure 8. GRP94 deficiency has no effect on some UPR targets.

(A) Representative Western blot results on the effect of GRP94 depletion on UPR targets. Grp94+/+ and −/− ESCs were treated with 300 nM Tg for the indicated time (hr). The whole cell lysates were subjected to Western blot to detect the level of EDEM, PERK phosphorylation, total PERK, CHOP and p62. (B) The same as in (A) with Tg treatment for 0, 4 and 16 hr. The level of eIF2α phosphorylation, total eIF2α, ATF4 and HSP70 were measured by Western blot. These experiments were repeated two to four times. (C) The levels of p-PERK and p-eIF2α were quantitated and normalized against total PERK and eIF2α, respectively. The data are presented as mean±s.e.m. The experiments were repeated two to four times.