Figure 7.
Effect of the amount of E-selectin added to E-selectin/anti-EpCAM mixture on rolling velocity of HL-60 cells and capture efficiency of DsRED-MCF-7 cells. Mixture of the two cell populations (1:1) were injected onto the surfaces co-immobilized with anti-EpCAM and E-selectin under the presence of anti-IgG at a shear stress of 0.16 dyn/cm2. The amount of immobilized E-selectin was increased from 0, 0.3, and 1.5 to 7.5 μg, while the amount of immobilized anti-EpCAM was constant at 1.5 μg. The rolling velocities of HL-60 cells on each slides were 4.74 ± 0.32 (0.3 μg), 1.82 ± 0.10 (1.5 μg), and 0.07 ± 0.12 (7.5 μg of E-selectin) μm/sec. Error bars: standard error.