Fig. 3.

Topical application of ETA prevents UV-induced interleukin-1beta (IL-1β) and cyclooxygenase-2 (COX-2) expressions in hairless mouse skin in vivo. Female HR-1 hairless mice were topically treated with vehicle (ethanol:polyethylene glycol=30:70) only, 0.1% ETA or 1% ETA once a day for 3 successive days after one time irradiation of UV (200 mJ/cm²) on dorsal skins. Skin biopsy was carried out on the fourth day (72 hr after UV irradiation). (A) Expression of IL-1β mRNA was determined by quantitative real-time RT-PCR. (B) Expression of IL-1β protein was analyzed by ELISA. (C) Expression of COX-2 mRNA was detected by quantitative real-time RT-PCR. (D) Expression of COX-2 protein was detected by Western blot analysis. Results are expressed as fold change. Values are mean±SEM (n=8).

^*P<0.01, ^†P<0.001 vs. control (CON) vehicle group; ^‡P<0.05, ^§P<0.01 ^∥P<0.001 vs. UV-irradiated (UV) vehicle group.