Figure 4.

Regulation of tryptase transcriptional activity via CACCTG motifs (E-boxes). (A) Diagrams show point mutations of MITF-binding sites in the tryptase promoter constructs used for the luciferase activity assay. (B) To examine beta-tryptase promoter activity via two E-boxes, mutations of MITF-binding sites were constructed by PCR and inserted into upstream of luciferase gene in pGL3-basic plasmid. The reporter plasmids were introduced into HMC-1 cells, and the cells were incubated for 40 h before luciferase activity assay.