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. 2010 Mar 8;42(5):366–375. doi: 10.3858/emm.2010.42.5.038

Figure 6.

Figure 6

The increased expression of tryptase by MITF overexpression in HMC-1 cells. (A) After transfection of MITF-A (0.1 or 1 µg) or empty expression vector (1 µg) into HMC-1 cells, the expression of MITF protein were analyzed by western blot analysis using anti-human MITF monoclonal antibody. (B) The expression of tryptase mRNA was analyzed using RT-PCR, and (C) tryptase enzymatic activity was measured using a substrate tosyl-gly-pro-lys-pNA (Sigma) using precision microplate reader, as described in the Methods.