Figure 1. JAM-C expression is restricted to specific epithelial cell lines.
(A) Using RT–PCR, JAM-C mRNA is variably expressed in epithelial cell lines. As positive controls, platelets and SK-CO15/JAM-C were used. GAPDH was used as loading control. (B) Using ELISA assay, JAM-C antibodies were tested to specifically recognize the recombinant protein JAM-C/hFc or other human recombinant proteins of the JAM family or structurally similar proteins. BSA-coated wells in the absence of recombinant protein were used as control.