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. Author manuscript; available in PMC: 2011 Jun 15.
Published in final edited form as: Prostate. 2010 Jun 15;70(9):982–992. doi: 10.1002/pros.21132

Figure 3. Snail inhibits cell proliferation in transfected LNCaP cells, but conditioned media from Snail-transfected cells can increase cell proliferation of parental LNCaP and PC3 cells by a paracrine mechanism.

Figure 3

(A) LNCaP-Neo1 and LNCaP-Snail1 or LNCaP-Neo5 and LNCaP-Snail5 clones were plated onto 96-well plates and MTS assay performed for 0–3 days. Cells transfected with Snail showed decreased cell proliferation. (B) Parental LNCaP or PC3 cells were plated overnight onto 96-well plates, followed by replacement of media with 25% conditioned media from either LNCaP-Neo1 or LNCaP-Snail1 cells. The MTS assay was then performed for 0–3 days. All experiment were done in triplicate and repeated twice independently. Bars, SD *, P < 0.05, Student's t test compared with LNCaP-Neo1 CM.