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. Author manuscript; available in PMC: 2011 Jun 15.
Published in final edited form as: Prostate. 2010 Jun 15;70(9):982–992. doi: 10.1002/pros.21132

Figure 6. Paracrine cell proliferation induced in LNCaP and PC3 parental cells by LNCaP-Snail conditioned media can be abrogated by NSE and CgA siRNA.

Figure 6

(A) LNCaP-Snail5 cells were plated in 10% FBS overnight then transfected with either 200 nM control siRNA or 200 nM NSE and/or CgA siRNA for 7 days. Untreated LNCaP-Neo5 and LNCaP-Snail5 were included as controls. Total cell lysates were prepared and analyzed by western blot analyses for Snail, NSE and CgA. Actin was utilized as a loading control for western blot. (B) Quantification of the Western blot results by densitometry with normalization to actin levels was done using the Quantity One quantification software (BioRad). The conditioned media from untreated LNCaP-Neo5, untreated LNCaP-Snail5 and LNCaP-Snail5 cells treated for 7 days with either control or NSE/CgA siRNA was collected and added to (C) parental LNCaP, or (D) PC3 cells whose cell proliferation was monitored for up to 4 days. All experiments were done in triplicate and repeated twice independently. Bars, SD, P = 0.069 for LNCaP treated with NSE+CgA siRNA CM for 4 days, P = 0.058 for PC3 treated with, NSE+CgACM for 4 days; Student's t test compared with control siRNA CM.