Table 1.
Comparison of blood parameters between mice euthanized by using CO2 or ketamine–xylazine
| CO2 group |
Ketamine–xylazine group |
||||||
| n | Mean (1 SD) | Median (range) | n | Mean (1 SD) | Median (range) | Pa | |
| RAPIDlab 1265 analyzer | |||||||
| pH | 12 | 6.9 (0.1) | 6.9 (6.8–7.0) | 16 | 7.2 (0.1) | 7.2 (7.1–7.3) | 0.0001 |
| pCO2 (mm Hg) | 11 | 153.3 (38.8) | 169.5 (76.9–200.9) | 15 | 61.6 (14.8) | 61.8 (36.9–86.3) | 0.0003 |
| Na+ (mmol/L) | 12 | 152.5 (2.7) | 151.9 (150.2–159.5) | 16 | 141.2 (3.9) | 141.7 (134.9–146.2) | 0.0001 |
| Ca+ (mg/dL) | 12 | 5.4 (0.3) | 5.3 (4.8–5.9) | 16 | 4.0 (1.0) | 4.4 (2.1–5.1) | 0.0002 |
| Glucose (mg/dL) | 11 | 167.9 (51.0) | 174.0 (58.0–257.0) | 15 | 265.1 (43.0) | 260.0 (223.0–387.0) | 0.0007 |
| Lactate (mmol/L) | 12 | 3.9 (0.9) | 4.0 (1.8–5.3) | 14 | 2.1 (0.8) | 2.0 (1.1–3.6) | 0.0020 |
| Cl− (mmol/L) | 12 | 111.9 (4.1) | 111.0 (107.0–121.0) | 15 | 113.3 (3.3) | 113.0 (105.0–118.0) | 0.1700 |
| HCO3− (mmol/L) | 12 | 25.0 (10.2) | 28.7 (3.6–34.9) | 15 | 23.1 (3.2) | 24.1 (17.0–27.1) | 0.1400 |
| Anion gap (mEq/L) | 12 | 22.5 (7.5) | 18.2 (15.5–37.3) | 15 | 8.8 (5.8) | 8.6 (–2.2–23.4) | 0.0004 |
| Plasma K+ (mmol/L) | 12 | 6.9 (0.7) | 6.9 (6.0–8.3) | 16 | 4.2 (0.6) | 4.2 (3.5–5.3) | 0.0001 |
| Olympus AU400 analyzer | |||||||
| Serum K+ (mmol/L) | 38 | 7.0 (0.5) | 7.0 (6.0–8.1) | 8 | 3.8 (0.3) | 3.8 (3.5–4.6) | <0.0001 |
a
Mean values of the same parameter were compared.