Figure 3.

Force-dependent unbinding of digoxigenin and its antibody. Force clamps ranging from hundreds of femtoNewtons to several picoNewtons were applied using the CFM (solid triangles), as well as with the optical trap (open triangles). Each CFM data point was obtained from a single experiment lasting a few minutes, whereas optical trap data was collected serially over a period of many hours. Histograms of the rupture times with a 20 s bin width (10 s for the highest optical trap force) were fit with a decaying exponential to obtain the off-rate at each force (Inset). Plotted error bars in the off-rate result from the uncertainty in the least squares fit.