Figure 4.

A total of 33,600 compounds were selected from a donated Biogen Idec library to represent the overall chemical diversity of the library and were screened using the phosphomolybdate blue ATPase assay. Reactions proceeded for 35 min at 37 °C and contained RecA (0.5 μM), poly(dT) (5 μM-nts), ATP (0.75 mM), and assessed compounds (final concentration 17 μM). In the upper panel, the screening results are represented as a scatter plot of relative inhibition (%) effected by each compound. There were 73 primary hits above a threshold of 50% inhibition. In the lower panel, a frequency distribution of the assay results is depicted as a histogram, where the number of compounds in each bin (± 5% width) is plotted on a logarithmic scale.