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. Author manuscript; available in PMC: 2010 Aug 25.
Published in final edited form as: Nature. 2010 Feb 25;463(7284):1048–1053. doi: 10.1038/nature08787

Figure 3. ORM gene function is conserved in human cells.

Figure 3

a, HEK293T cells were transfected with either an empty vector or a vector encoding Ormdl3 fused to the 3×Flag epitope (N- or C-terminal fusion). Immunoprecipitations with anti-Flag agarose were analyzed by Western blot against human serine palmitoyltransferase (α-hSptlc1) and against the Flag epitope (α-Flag). b, Hela cells were transfected with siRNAs directed against the indicated genes. After 72 hr, cells were harvested and their lipids were analyzed by mass spectrometry. Ceramide levels normalized to phosphatidylcholine (PC) are shown (relative to untreated cells; average ± s.d., n = 3).