. 2010 Jun;82(6):1041–1046. doi: 10.4269/ajtmh.2010.09-0691

Table 2.

Summary of markers used for genotyping T. gondii

Marker (location)	External primers	Internal primers	Restriction enzymes
SAG2 (VIII)5′3′¹⁵	F4: GCTACCTCGAACAGGAACAC	F: GAAATGTTTCAGGTTGCTGC	Sau 3AI
	R4: GCATCAACAGTCTTCGTTGC	R2: GCAAGAGCGAACTTGAACAC	Sau 3AI
	F3: TCTGTTCTCCGAAGTGACTCC	F2: ATTCTCATGCCTCCGCTTC	HhaI
	R3: TCAAAGCGTGCATTATCGC	R: AACGTTTCACGAAGGCACAC	HhaI
SAG3 (XII)¹²,¹⁶	P43S1: AACTCTCACCATTCCACCC	P43AS2: TCTTGTCGGGTGTTCACTCA	Nci I
SAG3 (XII)¹²,¹⁶	P43AS1: GCGCGTTGTTAGACAAGACA	P43S2: CACAAGGAGACCGAGAAGGA	Nci I
GRA6 (X)	GRA6-F1x: ATTTGTGTTTCCGAGCAGGT	GRA6-F1: TTTCCGAGCAGGTGACCT	Mse I
GRA6 (X)	GRA6-R1: GCACCTTCGCTTGTGGTT	GRA6-R1x: TCGCCGAAGAGTTGACATAG	Mse I
BTUB (IX)	Btb (ext) F: TCCAAAATGAGAGAAATCGT	Btb-F: GAGGTCATCTCGGACGAACA	Taq I BsiE I
BTUB (IX)	Btb (ext) R: AAATTGAAATGACCGAAGAA	Btb-R: TTGTAGGAACACCCCGACGC	Taq I BsiE I
APICO (plastid)	Apico-oF: TGGTTTTAACCCTAGATTGTGG^*	Apico-F: TGCAAATTCTTGAATTCTCAGTT	Afl II Dde I
APICO (plastid)	Apico-oR: AAACGGAATTAATGAGATTTGAACT^*	Apico-R: GGGATTCGAACCCTTGATA	Afl II Dde I

This study.