Figure 2.

The 5′-nucleotidase/apyrase family of black flies, mosquitoes, and prokaryotes. (A) Clustal alignment. The Simulium nigrimanum proteins are Sim-214 and Sim187. The remaining sequences are named with the first three letters from the genus name followed by two letters from the species name and by their National Center for Biotechnology Information (NCBI) protein accession number. The symbols above the alignment indicate: (*) identical sites; (:) conserved sites; (.) less conserved sites. (B) Neighbor joining bootstrapped phylogram of the alignment in A, showing 4 clades. The numbers on the branches represent the percent bootstrap support. The bar in the bottom represents 10% amino acid divergence. Mosquito proteins are indicated by a triangle, Simulium proteins by a square, and bacterial proteins by a circle. For more details, see text. This figure appears in color at www.ajtmh.org.