Figure 5.

The Simulium D7 salivary protein family. Phylogram deriving from the alignment of S. nigrimanum proteins (starting with Sim-) with those of Simulium vittatum, indicated by SIMVI_X where X is their National Center for Biotechnology Information (NCBI) accession numbers. The numbers on the tree bifurcations indicate the percentage bootstrap support above 75%. The bar at the bottom represents 20% amino acid substitution. Members of the short D7 family are marked with a circle, those from the long family with a square. Protein sequences were aligned by the Clustal program39 and the dendrogram was done with the Mega package41 after 10,000 bootstraps with the neighbor joining (NJ) algorithm. For more details, see text. This figure appears in color at www.ajtmh.org.