Figure 8.

The Simulium vittatum erythema protein (SVEP) superfamily of Simulium. (A) Clustal aligment. The symbols above the alignment indicate: (*) identical sites; (:) conserved sites; (.) less conserved sites. (B) Bootstrapped phylogram. The Simulium nigrimanum proteins are indicated by a square. The S. vittatum sequences are named SIMVI followed by their National Center for Biotechnology Information (NCBI) protein accession number. The vasodilator SVEP is marked with a triangle. The numbers on the tree bifurcations indicate the percentage bootstrap support. The bar at the bottom represents 10% amino acid substitution. Protein sequences were aligned by the Clustal program39 and the dendrogram was done with the Mega package41 after 10,000 bootstraps with the neighbor joining (NJ) algorithm. For more details, see text. This figure appears in color at www.ajtmh.org.