Table 2.

Median maximum likelihood estimator values, minimum and maximum values, and percentage of maximum likelihood estimator values under the population infection rate (percentage underestimations) for 32 frequency distributions calculated for hypothetical mosquito populations with viral titer Distribution 1

Population infection rate	Sample size	Pool size	Low virus detection ability			High virus detection ability
			MLE	Minimum–maximum	% Underestimation	MLE	Minimum–maximum	% Underestimation
1/1,000	200	20	0	0	100	0	0–10.51	99.8
		50	0	0	100	0	0–11.35	99.9
	2,000	20	0	0	100	0.50	0–2.03	82.6
		50	0	0	100	0.50	0–2.08	82.3
5/1,000	200	20	0	0	100	0	0–16.68	91.9
		50	0	0	100	0	0–20.20	92.8
	2,000	20	0	0–0.50	100	2.03	0–6.90	98.5
		50	0	0–0.50	100	2.08	0–7.71	97.8
10/1,000	200	20	0	0–4.99	100	4.99	0–23.67	81.5
		50	0	0–4.93	100	4.93	0–20.20	83.2
	2,000	20	0	0–0.50	100	4.14	0–10.43	99.8
		50	0	0–0.50	100	4.39	0–10	99.9
15/1,000	200	20	0	0	100	4.99	0–31.77	97.3
		50	0	0–4.93	100	4.93	0–20.20*	98.0
	2,000	20	0	0–0.50	100	4.14	0.5–9.23	100
		50	0	0–0.50	100	4.39	0.5–10.83	100

^*Two of the samples produced all positive pools and were excluded because maximum likelihood estimator (MLE) values cannot be calculated with all positive pools.